Journal: bioRxiv

Article Title: Mechanical Ventilation induced DNA Damage and P21 in an Acute Aging Model of Lung Injury

doi: 10.1101/2022.03.08.483505

Figure Lengend Snippet: Immunofluorescence staining of lung section for both young and old mice. Staining for KRT8 (red), a marker of transiently differentiated AT2, and SFTPC (green), an AT2 marker. DAPI (blue) was used as a counterstain. The staining reveals an increase in KRT8 positive cells with age and mechanical ventilation and a decrease in SFTPC in all groups compared to young non-ventilated. NV: non-ventilated and HP: high pressure.

Article Snippet: Primary antibodies: mouse anti-gamma H2AX (Novus Biologicals, NB100-74435), rabbit anti-Ki67/MKi67 (Novus Biologicals, NB500-170SS), rabbit anti-SP-C (Bioss, bs-10067R), rat anti-KRT8 (CreativeBiolabs, CBDH1469).

Techniques: Immunofluorescence, Staining, Marker

Journal: Journal of integrative neuroscience

Article Title: Kv1.3 Blockade Alleviates White Matter Injury through Reshaping M1/M2 Phenotypes via the NF-κB Signaling Pathway after Intracerebral Hemorrhage.

doi: 10.31083/j.jin2206171

Figure Lengend Snippet: Fig. 9. Application of PAP-1 inhibiting NF-κB activation after ICH. (A) Bar charts showing NF-κB P65 mRNA expression in each group on day 3 after ICH. n = 3 each group, ∗∗p < 0.01 vs. Sham group; #p < 0.05 vs. ICH group. (B) Bar charts showing NF-κB

Article Snippet: Then the sample was incubated separately with the following primary antibodies overnight at 4 °C: Rabbit anti-Kv1.3 (1:1000, No.14079-1-AP, Proteintech, Philadelphia, PA, USA); Rabbit anti-MBP (1:1000, No.BA0094, Boster, Wuhan, Hubei, China); Rabbit antiCD16 (1:1000, No.16559-1-AP, Proteintech, Philadelphia, PA, USA); Rabbit anti-CD206 (1:1000, No.18704-1-AP Proteintech, Philadelphia, PA, USA); Rabbit anti-iNOS (1:1000, No.22226-1-AP, Proteintech, Philadelphia, PA, USA); Rabbit anti-Arg-1 (1:1000, No.16001-1-AP, Proteintech, Philadelphia, PA, USA); Mouse anti-TNF-α (1:1000, No.sc-52746, Santa Cruz, Dallas, TX, USA); Mouse anti-IL-1β (1:1000, No.sc-32294, Santa Cruz, Dallas, TX, USA); Rabbit anti-IL-4 (1:1000, No.66142-1Ig, Proteintech, Philadelphia, PA, USA); Rabbit anti-IL10 (1:1000, No.60269-1-Ig, Proteintech, Philadelphia, PA, USA); Mouse anti-NF-κB p65 (1:200, No.sc-8008, Santa Cruz, Dallas, TX, USA); Mouse anti-NF-κB p50 (1:200, No.sc-8414, Santa Cruz, Dallas, TX, USA); Mouse antip-NF-κB p65 (1:200, No.sc-136548, Santa Cruz, Dallas, TX, USA); Mouse anti-p-NF-κB p50 (1:200, No.sc271908, Santa Cruz, Dallas, TX, USA); Mouse anti-βTubulin (1:5000, No.T200608, Zen-bio, Chengdu, Sichuan, China); Mouse anti-GAPDH (1:5000, No.250133, Zen-bio, Chengdu, Sichuan, China); Mouse anti-β-Actin (1:5000, No.700068, Zen-bio, Chengdu, Sichuan, China).

Techniques: Activation Assay, Expressing